A young woman with exceptional skills who carries on the name of the famous Yagyū clan, possessing the rare Demon Eye of the Oni trait. Prior to the beginning of the game itself, Akane is assigned the duty of hunting down Munenori Yagyū to restore her clan's honor.
Assuming the name of Jubei, Akane hears of Soki's deeds and finds him at the Plains Outpost at Suruga to defeat him. But after losing to him, she joins him in his fight against the Genma. Near the end of the game, she is depicted wearing an eye patch to cover her Oni Eye. At one point in the game, Akane is confused as to whether she is romantically interested in Sōki, or he is just her elder 'brother'. A scene after the end credits depicts her starting off on another journey, as she has heard rumors that "someone is still alive".
- Akane's swift, iaijutsu-like style of attack is similar to that of Kaede from Onimusha: Warlords.
- Shikomizue swordsticks are her main weapons. Akane Yagyu is the daughter of Yagyu Munetoshi's first son Yagyu Toshikatsu, noted by virtue of her knowledge of Yagyu Seigo-ryu, the style that was incorporated into her brother, Yagyu Toshiyoshi's Owari branch of Yagyu Shinkage-ryu.
- Akane made a cameo appearance in Soki's Tatsunoko vs. Capcom ending.
- Akane appeared in Onimusha Soul.