The game was re-released as part of the Phoenix Wright: Ace Attorney Trilogy game for the 3DS in 2014.
- Phoenix Wright
- Miles Edgeworth
- Dick Gumshoe
- Maya Fey
- Pearl Fey
- Franziska von Karma
- Mia Fey
- The judge
- Dahlia Hawthorne - Wright's former girlfriend.
- Winston Payne - a smug prosecutor who starts his cases with the upper hand on his side, but easily falls once a hole is blown.
Episode 1: Turnabout Memories - Mia Fey's second case as a defense attorney, in which she defends Phoenix, a University student at that time, for the death of another student. The prosecutor for this case is Winston Payne.
Episode 2: The Stolen Turnabout - Phoenix defends Ron DeLite, accused of being a famous thief, and later for the murder of Kane Bullard, the CEO of KB Security. It is also in this case where Phoenix meets Godot, who is the prosecutor, for the first time.
Episode 3: Recipe for Turnabout - Phoenix defends Maggey Byrde once more, this time for the murder of a client at the restaurant where she works. Prosecutor for the case is Godot, but Payne was the prosecutor for the trial that occured on the month before.
Episode 4: Turnabout Beginnings - Phoenix looks up a file that follows Mia Fey's first case, in which she faces Miles Edgeworth in his first case as prosecutor. It is chronologically the earliest playable case in the series. Terry Fawles spends five years in prison for the murder of a woman who faked her own death. Mia Fey defends Terry Fawles for being accused of Valerie Hawthorne's death after escaping from prison. Diego Armando is also present her as Mia Fey's partner in court.
Episode 5: Bridge to the Turnabout - Miles Edgeworth and Phoenix defend the mysterious nun, Iris, for the death of the enigmatic Elise Deauxnim, a children's picture book author. Prosecutor for the case is Franziska von Karma on the first day, then Godot on the second.